Stretch-activated signaling of nerve growth factor secretion in bladder and vascular smooth muscle cells from hypertensive and hyperactive rats

2000 ◽  
Vol 183 (3) ◽  
pp. 289-300 ◽  
Author(s):  
David B. Clemow ◽  
William D. Steers ◽  
Jeremy B. Tuttle
Keyword(s):  
Smooth Muscle ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Nerve Growth ◽  
Factor Secretion ◽  
Growth Factor Secretion

scholarly journals Human iPSC-Derived Vascular Smooth Muscle Cells in a Fibronectin Functionalized Collagen Hydrogel Augment Endothelial Cell Morphogenesis

2021 ◽  
Vol 8 (12) ◽  
pp. 223
Author(s):  
Kaiti Duan ◽  
Biraja C. Dash ◽  
Daniel C. Sasson ◽  
Sara Islam ◽  
Jackson Parker ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Growth Factor ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Mechanistic Study ◽  
Αvβ3 Integrin ◽  
Factor Secretion ◽  
Growth Factor Secretion

Tissue-engineered constructs have immense potential as autologous grafts for wound healing. Despite the rapid advancement in fabrication technology, the major limitation is controlling angiogenesis within these constructs to form a vascular network. Here, we aimed to develop a 3D hydrogel that can regulate angiogenesis. We tested the effect of fibronectin and vascular smooth muscle cells derived from human induced pluripotent stem cells (hiPSC-VSMC) on the morphogenesis of endothelial cells. The results demonstrate that fibronectin increases the number of EC networks. However, hiPSC-VSMC in the hydrogel further substantiated the number and size of EC networks by vascular endothelial growth factor and basic fibroblast growth factor secretion. A mechanistic study shows that blocking αvβ3 integrin signaling between hiPSC-VSMC and fibronectin impacts the EC network formation via reduced cell viability and proangiogenic growth factor secretion. Collectively, this study set forth initial design criteria in developing an improved pre-vascularized construct.

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